The Fc (IgG1): FcRn Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling neutralizing antibodies or inhibitors of the interaction between Fc (IgG1) and human FcRn. This kit comes in a convenient 96-well format, with purified Biotinylated-FcRn complex (Fc receptor amino acids 24-297 and B2M amino acids 21-119) and Fc (IgG1) (amino acids 100-330) proteins, Streptavidin-HRP, and assay buffers for 100 reactions.

The assay mechanism is described next. Fc (IgG1) is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-FcRn, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.