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TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

數(shù)量:1供應(yīng)商:BPSBioscience細(xì)胞類(lèi)型:穩(wěn)轉(zhuǎn)細(xì)胞系品系:Jurkat(cloneE6-1)

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2023-10-12
  • 訪  問(wèn)  量:171
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詳細(xì)介紹
  • 數(shù)量:

    1

  • 供應(yīng)商:

    BPS Bioscience

  • 細(xì)胞類(lèi)型:

    穩(wěn)轉(zhuǎn)細(xì)胞系

  • 品系:

    Jurkat (clone E6-1), human T lymphoblast, suspension

  • 組織來(lái)源:

    /

  • 相關(guān)疾病:

    多種

  • 物種來(lái)源:

    human

  • 免疫類(lèi)型:

    多種

  • 器官來(lái)源:

    無(wú)

  • 運(yùn)輸方式:

    干冰

  • 年限:

    10

  • 生長(zhǎng)狀態(tài):

    良好

  • CAS號(hào):

    無(wú)

  • 英文名:

    TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

  • 注冊(cè)證號(hào):

    /

  • 規(guī)格:

    2管

This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.

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